Recombinant DNA technology involves which sequence of steps?

Recombinant DNA Technology Involves Which Sequence Of Steps?

Answers

Answer 1
In standard cloning protocols, the cloning of any DNA fragment essentially involves seven steps: (1) Choice of host organism and cloning vector, (2) Preparation of vector DNA, (3) Preparation of DNA to be cloned, (4) Creation of recombinant DNA, (5) Introduction of recombinant DNA into the host organism, (6) Selection
Answer 2

Answer:

B

Explanation:

Processes of recombinant DNA technology involve isolation of DNA of the desired DNA fragment. In order to cut the DNA with restriction enzymes, it needs to be in pure form, free from other macromolecules. The DNA is enclosed within the membranes, we have to break the cell open to release DNA along with other macromolecules such as RNA, proteins, polysaccharides, and also lipids. This can be achieved by treating the bacterial cells/plant or animal tissue with enzymes such as lysozyme (bacteria), cellulase (plant cells), chitinase (fungus). The RNA can be removed by treatment with ribonuclease whereas proteins can be removed by treatment with protease.


Related Questions

Your cells have a unique MHC (major histocompatibility complex). Directions for producing MHCs come from _________________.


A. the helper T-cells

B. the thymus

C. inherited DNA

D. the bone marrow

Answers

C. inherited DNA :)))

The directions for generating MHC comes from the option c. inherited DNA. hence, the option c is correct.

What is MHC?

The full form is a major histocompatibility complex. It is the genes group that have the proteins code that should be founded on the cell surface which helped the immune system, that recorded in the foreign substance.

So at the time of directing, it produced MHC that came from inherited DNA.

hence, the option c is correct.

learn more about DNA here: https://brainly.com/question/19739527

Do you think the organisms in image 3 support the theory that modern organisms evolved from ancestral organisms? Explain your answer.

Answers

Answer:

occurs when these heritable differences become more common or rare in a population, either non-randomly through natural selection or randomly through genetic drift.

Answer:Yes, the organisms in image 3 support the theory that modern organisms developed from ancestral organisms. The two organisms share similar body structures and traits. So, there will be similarities in their genes, and they’ll share an evolutionary connection.

Explanation: Edmentum answer

HELP I NEED HELP ASAP HELP I NEED HELP ASAP HELP I NEED HELP ASAP HELP I NEED HELP ASAP HELP I NEED HELP ASAP

Answers

I think it would be A

tru or false

Do plants lose water

Answers

Answer:

True is the answer of the question

Answer:

True they lose water by the process of Transpiration.

Explanation:

Hope this helped :)

30 POINTS!!! PLZZZ HELP
1. What processes create igneous rocks?
2. What processes create metamorphic rocks?
3. What processes create sedimentary rocks?

Answers

Answer:

Igneous rocks form when magma (molten rock) cools and crystallizes, either at volcanoes on the surface of the Earth or while the melted rock is still inside the crust.

Sedimentary rocks are erosion, weathering, dissolution, precipitation, and lithification. Erosion and weathering include the effects of wind and rain, which slowly break down large rocks into smaller ones.

Metamorphic rocks form when rocks are subjected to high heat, high pressure, hot mineral-rich fluids or, more commonly, some combination of these factors.

Explanation:

Answer:

1. Igneous rocks form when magma molten rocks cools and crystallizes, either at volcanoes on the surface of the Earth or while the melted rock is still inside the crust. All magma develops underground, in the lower crust or upper mantle, because of the intense heat there.

2. Metamorphic rocks form when rocks are subjected to high heat, high pressure, hot mineral-rich fluids or, more commonly, some combination of these factors. Conditions like these are found deep within the Earth or where tectonic plates meet.

3. The most important geological processes that lead to the creation of sedimentary rocks are erosion, weathering, dissolution, precipitation, and lithification. Erosion and weathering include the effects of wind and rain, which slowly break down large rocks into smaller ones.

Hope it Help!~

A fluke is a small animal that lives in the gut of mammals and takes energy from the mammal. A fluke is a
A. hunter.
B. decomposer.
C. parasite.
D, herbivore.

Answers

Answer:

id say its a parasite

Explanation:

Which conclusion can you draw from the information shown in the graph?
A. Carbon dioxide levels are currently much lower than normal.
B. Carbon dioxide levels are currently higher than they have ever
been.
C. Carbon dioxide levels in the past have been as high as they are
now.
D. Carbon dioxide levels are always about the same.

Answers

B. Carbon dioxide levels are currently higher than ever

Answer:

The answer is B. Here is proof. Plz mark brainliest

Explanation:

Explain what would occur if the population of an animal such as a beaver, changes the environment and can increase the number of individuals in that area. In other words, explain how a change to an environment can cause an increase in an animal population.

Answers

Answer: Some changes may cause a population to increase. If there are more plants than usual in an area, the population of animals that eat plants may increase. If one animal's population increases, the population of animals that eat that animal might also increase.

Explanation:

Two species of three-spined stickleback fish have been found together in five different lakes. The larger, bottom-dwelling species feeds on large prey close to shore. A surface-dwelling species feeds on small plankton in open water. DNA analysis shows that the two species in each lake are more closely related to each other than they are to any of the other species in the lake. Which prezygotic mechanism most likely allowed divergence of an ancestral species into these present-day species

Answers

Answer:

The answer is "Sympatric speciation due to disruptive selection"

Explanation:

Disruptive selection is a certain type of natural choice, that aggressively favors all ends of the spectrum in a moderate community. Disruptive selection is often deduced by a phyletic prepared to change to contribute to speciation. These two species happened due to various habitats associated with extreme speciation due to various confusing choices.

which igneous rock could physically weather to beach sand that contains the minerals pyroxene, plagioclase, feldspar, and olivine?

Answers

Answer:

gabbro

Explanation:

just took the test!

An igneous rock which could physically weather to beach sand that contains the minerals pyroxene, plagioclase, feldspar, and olivine is: Gabbro.

Weathering refers to both the chemical and physical breakdown of a rock into smaller fragments (pieces) referred to as sediments.

Generally, there are two (2) main types of weathering and these are;

1. Chemical weathering.

2. Physical weathering.

Gabbro is a coarse-grained (phaneritic), mafic intrusive type of igneous rock which physically weather to beach sand containing the following sediments or minerals:

Pyroxene.Plagioclase.Feldspar.Olivine.

Read more: https://brainly.com/question/21975453

4) A "black light" uses _______ radiation to make some objects glow.

5) How does a prism separate white light into the different colors that compose it?​

Answers

Answer:

4 Black light uses Ultraviolet light

5 As light passes through a prism, it is bent, or refracted, by the angles and plane faces of the prism and each wavelength of light is refracted by a slightly different amount. ... As a result, all of the colors in the white light of the sun separate into the individual bands of color characteristic of a rainbow.

Answer:

ultra violet light

Which two characteristics do all forces have?
O motion and size
O size and direction
O direction and speed
O balance and direction

Answers

Answer:

direction and speed are two characteristics of forces

PLEASE HELP FAST NEED FAST =)

3) Select the reasons why global warming might still occur even if greenhouse-gas emissions drastically decrease.

Answers

Answer:

If the emission of greenhouse gases were stopped, global warming would still happen due to the gases that are already in the atmosphere and the constant deforestation.

Explanation:

Greenhouse gases are the biggest creators of global warming. This is because these gases cause holes in the ozone layer, which protects the planet from the sun's rays. These holes cause the sun's rays to reach the earth's surface with greater intensity, promoting global warming.

Trees are able to absorb these gases, decreasing their concentration in the atmosphere and preventing them from degrading the ozone layer. However, the constant deforestation has reduced the number of plants and the remaining amount on the planet cannot cope with the constant emission of these gases. In this case, even if the emissions of greenhouse gases were ended, the amount of these gases in the atmosphere would still be very large. Plants were slow to absorb all these gases, which would not prevent global warming from happening.

please help it’s due soon!!

Answers

Answer:

A - Muscle

B - Tendon

C - Cartilage

D- Ligament

E- Bone

Explanation:

The above are the parts of a musculoskeletal system.

The musculoskeletal system is actually known as an organ system which is made up of specialized tissues of the bones and skeletal muscles. The system consists of the bones of the body, muscles, ligaments, cartilage, tendons, joints and other connective tissues. These all work together in the musculoskeletal system to provide support for the body. They enable motion of the body and provide protection to vital organs in the body.

A gene has 2 alleles, R & B, List the possible genotypes? I also need help with questions 5, 6, 7 and 8 please help! Pls look at the picture! I will mark whoever sounds best the brain! Ty!

Answers

Answer:

5.) The possible genotypes should be: RR RB BB ( if the could for the same proteins)

6.) Frequency of R: 0.5

7.)Frequency of R: 0.5

8.)Frequency of R: 0.375

Explanation:

5.) If R and B code for the different forms of the same protein then they are only a few possible out comes

6.) First calculate the total alleles in population

allele R= 40

Allele B=40

total allele = R+B= 40+40

=80

Now to find allele R frequency is:

(Total R alleles) / (Total allele in pop)

40/80=

0.5

7.)Calculate the total alleles

Alleles from for R

RR= 10

R=10x 2= 20

Multiply the value by 2 because there are 2 R alleles present in

RB=20

R=20

Number doesn't change there is only 1 R allele

Total R=20+20

=40

Alleles For B

BB=10

B=10×2

=20

Same thing here, two B alleles together so multiple by 2

RB=20

B=20

Total B= 20+20

=40

Total alleles in pop add

40+40

=80

Frequency of R

Total of R/Total Alleles

=40/80

=0.5

8.)Repeat the same thing in 7 but use different numbers

RR=10

R=10x2

=20

RB=10

R=10

Total R= 20+10

=30

Which of the following is the best definition of alternative energy?
A. Energy sources that can be used in place of hydroelectric energy
B. Energy sources that can be used in place of wind energy
C. Energy sources that can be used in place of coal
D. Energy sources that can be used in place of fossil fuels

Answers

Answer:

D

Explanation:

Alternative energy is best defined as the use of energy sources other than traditional fossil fuels, which are considered environmentally harmful and are in short supply. Fossil fuels consist of natural gas, coal, and oil. Currently, fossil fuels are the most used energy source to heat our homes and power our cars.

Answer:

D.)

Explanation:

Alternative energy is best defined as the use of energy sources other than traditional fossil fuels, which are considered environmental harmful and are in short supply.

how are bacterias different from animals ?

Answers

Answer:

Bacteria are neither animals nor plants.... Because bacteria are prokaryotic, they do not have a nucleus and no membrane-bound organelles. In contrast, plants and animals are made up of eukaryotic cells, which means they have a nucleus and membrane-bound organelles like mitochondria or golgi apparatus.

Explanation:

Answer:

Bacterias have simple cells and are typically single cell organisms where as animals which have more complex cells are are multi celled organisms UwU

HOPE THIS HELPS

-Todo <3

Explanation:

nombradme 5 artropodos que respiren mediante traqueas

Answers

Answer:

Basic Categories of Arthropods

Chelicerata: horseshoe crabs, spiders, scorpions, sea spiders and mites.

Myriapoda: millipedes, centipedes, pauropods and symphylans (known as glasshouse symphylans or garden centipedes)

Crustacea: brine shrimp, barnacles, lobsters, crabs, shrimp and remipedes.

Explanation:

Hope it helps

Please anyone help please please help ASAP ASAP please

Answers

Answer:

radiant to electrical

Explanation:

7.
How is the rate of transpiration affected by decreasing temperature and by decreasing light
intensity?
decreasing
temperature
decreasing
light intensity


A
slower
slower
B
slower
faster
С
faster
slower
D
faster
faster

Answers

Answer:

B

slower

faster

Explanation:

13. What type of chemical hazard is a person ingesting if they eat a lot of processed foods, cured

meats, and certain types of wines?

Answers

Answer:

The correct answer is - health hazards (carcinogenic).

Explanation:

Processed foods, cured meat, and certain types of wines have a chemical present in them for increasing the life of the food and taste. This chemical is used in this food are known for their cancer-causing properties and grouped as group 1 carcinogen which comes under health hazard of type of chemical hazard.

N-nitroso-compounds (NOC) and polycyclic aromatic hydrocarbons (PAH) are the two major chemicals used in cured meat and processed food. There are many other severe health conditions also caused by ingesting a high amount of such food like diabetes, cardiovascular disease, and obesity.

Chandra X-ray observatory has reported that they have found a new star near our solar system. The Chandra X-ray telescope detects x-rays in space. What does the use of the x-ray telescope review about the newly found star? ( PLS EXPLAIN Y THE ANSWER IS CORRECT)

a. The star produces electromagnetic waves with shorter wavelengths than visible light.
b. The star produces electromagnetic waves with lower frequencies than visible light.
c. The star produces pictures in addition to electromagnetic waves.
d. The star produces vibrating electrons

Answers

Answer:

It’s possibly b, or a but I am definitelyonly 49% sure

Explanation:

Hope this helped you Arianna!

Which of these protects water that is
between the United States and Canada?
A. Endangered Species Act
B. Superfund Act
C. Convention on the Protection/Use of Transboundary
Watercourses
D. London Convention on the Prevention of Marine
Pollution

Answers

Answer:

C. Convention on the Protection/Use of Transboundary

Watercourses

Explanation:

I think the answer is C.

Which of the following conditions is least likely to have an effect on natural selection in a species of sheep that are geographically isolated on an island?

A. available niches
B. existing predators
C. chromosome number
D. available food resources

Answers

Answer:

A. available niches

Hope it helps!

Answer:

C.

Chromosome number

Explanation:

In a human karyotype, the 46 chromosomes of the human genome are arranged into 23 pairs. Encyclopædia Britannica, Inc. An organism with any multiple diploid numbers of chromosomes is said to be polyploid.

First, describe why an animal is considered a system. Then, explain why an animal is not considered a technological system. ▲

Answers

A system is a group of organs that work together and provide an organism with an advantage for survival. It is the most complex organization in your body and the final level of the progression from cells to tissues to organs and then systems.

How do you think molecules of food rearrange when they react with oxygen in the cells of your body?​

Answers

Answer:

In cellular respiration, complex molecules containing carbon react with oxygen to produce carbon dioxide and other materials. Within individual organisms, food moves through a series of chemical reactions in which it is broken down and rearranged to form new molecules to support growth or to release energy.

Explanation:

The glucose and oxygen atoms are rearranged into carbon dioxide and water molecules during aerobic respiration. Being an exothermic chemical reaction, this process releases energy as it goes along.

What is aerobic respiration?

A chemical reaction that converts carbohydrates into energy using oxygen (sugars). also known as oxidative metabolism, aerobic metabolism, and cell respiration.

During aerobic cellular respiration, glucose and oxygen combine to create ATP, which the cell can utilise.

As byproducts, carbon dioxide and water are produced. The general equation for aerobic cellular respiration is: ATP is produced when glucose and oxygen combine in cellular respiration.

During aerobic respiration, glucose and oxygen atoms are rearranged into carbon dioxide and water molecules. As it proceeds, this process releases energy since it is an exothermic chemical reaction.

Thus, molecules of food rearrange when they react with oxygen in the cells of your body.

For more details aerobic respiration, visit:

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Organisms that eat other organisms are called __________.

Answers

Answer:

Heterotrophs Or Consumers

Answer:

heterotrophs  

Explanation:


Which of the following is the best example of a population?

Answers

Answer:

B) A school of sunfish.

In biology, a population is a number of all the organisms of the same group or species who live in a particular geographical area and are capable of interbreeding

Answer:B

Explanation:   JUST TOOK THE TEST TRUST ME!

what is the difference between the substrate and product?​

Answers

Answer:

The key difference between substrate and product is that substrate is the staring material of a chemical reaction whereas product is the compound obtained after the completion of the reaction. The terms substrate and product are used in spontaneous reactions and in reactions upon which an enzyme acts on as a catalyst.

Explanation:

Explanation:

substrate is a chemical reaction and product is compound obtained

10. Why are most species that have ever lived on Earth extinct?

Answers

Explanation:

At least 571 species have been lost since 1750, but likely many more. The main cause of the extinctions is the destruction of natural habitats by human activities, such as cutting down forests and converting land into fields for farming.

Answer:

Some animals have gone extinct due to over hunting on them for skin, while some have died of because they weren't able to adapt to their environment because it change over time, causing them to go extinct  

Explanation:

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I NEED HELP ASAP PLS!! Meagan and jake both live on the same street that the librarian is on.Megan lives 3.5 blicks north of the library,and jake lives 4.5 blocks south of the library.How many blpcks apart do jake and meagan live. What is the median of the data set shown?34, 86, 12, 56, 21, 98, 72, 34, 21, 34, 45, 23, 97, 44 Forms include compromise, truce, or mediation. answer truthfully:)) Which example describes an abiotic factor interacting with a biotic factor?Small fish are food for larger fish.More light increases the water temperature.Tropical fish need warm water to survive.High water temperatures decrease oxygen concentration in the water. How would the finch populations on these islands change over time? Suppose a program is supposed to reverse an array. For example, if we have the array arr = {1, 2, 3, 4, 5}, after reversing the array we would have arr = {5, 4, 3, 2, 1}.Most of the code for the program is shown here:Const int SIZE = 5;int arr[SIZE] = {1, 2, 3, 4, 5};int firstindex =0, lastindex = SIZE-1, temp;1 _______________________________{temp = arr[firstindex];arr[firstindex] = arr[lastindex];arr[lastindex] = temp;firstindex++;lastindex--;}All of the following options below could be correctly inserted at line 1 in the code shown above EXCEPT:a. while(firstindex < lastindex)b. while(firstindex what are the different types of solid waste? Explain them PLz plz plz Help will mark brainlist When seeking information about mental health it is important to use valid resources. Which of the following are items you should look for when determining if a source is valid?Group of answer choicesWho the author is and how are they qualifiedHow current the information isThe facts are cited or referencedall of these are important to review PLZ HELP ME IT IS INPORTANT PLZ THANKS HELP ME PLZZZZZZZZZZZZZ plz help first right gets brainly Contrast mechanical and chemical digestion. How was your trip? Tell me ____ it! if we use the quadratic expression below to complete a diamond, what value will go at every bottom of the diamonda. -4b. 4c. 1d. 5 Allegheny Company ended Year 1 with balances in Accounts Receivable and Allowance for Doubtful Accounts of $58,000 and $2,700, respectively. During Year 2, Allegheny wrote off $4,800 of Uncollectible Accounts. Using the percent of receivables method, Allegheny estimates that the ending Allowance for Doubtful Accounts balance should be $4,400. What amount will Allegheny report as Uncollectible Accounts Expense on its Year 2 income statement (a)Describe the FOUR (4) methods of allocating joint costs to products. (12 marks)(b)Weng Tai company simultaneously produces three products (X, Y and Z) from a single process. X and Y are processed further before they can be sold; Z is a by-product that is sold at split off point for RM2 per unit without incurring any further process costs. Joint costs are apportioned using the final sales value method. The sales prices of X and Y after further processing are RM40 per unit and RM35 per unit respectively. Data for April are as follows:Joint production costs that produced 5,000 units of X, 5,500 units of Y and 3,500 units of Z150,000Further processing costs for 5,000 units of X30,000Further processing costs for 5,500 units of Y40,000Required:Calculate the total costs and profit for the production of X and Y. (14 marks)(c)Discuss THREE (3) arguments in support of variable costing and absorption costing. (12 marks)(d)Zency Sdn Bhd produces pens. Data concerning the companys operations last year appear as follows:Units in beginning inventory5,000Units produced50,000Units sold46,000Selling price per unitRM2.50 Variable costs per unit:Direct materialsRM0.80 Direct laborRM0.50 Variable manufacturing overheadRM0.10 Variable selling and administrative overheadRM0.05 Fixed costs in total:Fixed manufacturing overheadRM20,000 Fixed selling and administrative overheadRM10,000 Required:Prepare an income statement for the year using absorption costing. (12 marks) Do I have the right answers Which translation vector moves every point of a pre-image 4 units left and 6 units up?F. (4, -6)G. (-4, 6)H. (-6, 4)J. (6, -4)