Explain the process of DNA isolation.

Answers

Answer 1

Answer:

Lysis, precipitation and purification

Explanation:

DNA isolation is a molecular biology technique based on the extraction of DNA and subsequent purification to remove inhibitors that may hamper the process of amplification. DNA isolation consists of three steps:

1- Lysis: it consists of the mechanical disruption of the cell, where the nucleus is broken to release DNA. During this process, it is required to use enzymes and detergents such as Proteinase K

2- Precipitation: during this process, the DNA is precipitated by using alcohol and salt.  

3- Purification: this process removes the impurities from DNA. Extraction is carried out by washing DNA with ethanol and water.


Related Questions

Is this right? NEED HELP ASAP

Answers

Answer:

They all seem to be correct, good job!

Answer:

Correct

Explanation:


A topographic map is a two-dimensional model that uses contour lines to represent points of equal
1
barometric pressure
2
temperature gradient
3.
elevation above sea level
4
magnetic force

Answers

Answer:

3. elevation

Explanation:


Write the complementary RNA strand to the following DNA sequence: 5'ACGTAC 3'

Answers

3’ UGCAUG 5’
hope this helps!

What is a function of the respiratory system?

Answers

Answer:

The respiratory system's main purpose is to move fresh air into your body and moving waste gasses out (like carbon dioxide when you breathe out)

The function of the respiratory system is to inhale oxygen which is absorbed by red blood cells in the lungs. The RBC then go to the heart which pumps the oxygenated blood to the rest of the body and non oxygenated blood goes back to lungs to be oxygenated. They also carry back waste such as carbon dioxide to the lungs to be exhaled.

It takes place when magma introduces
great amount of heat into an existing rock
resulting in the recrystallization and mineral
reaction in the rock.​

Answers

Answer:

The correct answer is - contact metamorphism.

Explanation:

Contact metamorphism is one of the two metamorphism processes which takes place when the pre-existing rock is introduced to a great amount of heat which affects it by an intrusion of magma. It is known as a low pressurized, high-temperature type of metamorphism.

An example of this type of metamorphism which experiences recrystallization in the rock and forms a metamorphic marble that is formed by the recrystallization and mineral reaction of the limestone.

I NEED HELP ASAP!! will be willing to mark you brainliest!
Answer Q1-7 using the food web pictured below.
1. How many food chains make up the food web?



2. Which organism is an herbivore?



3. Which organism is an autotroph?



4. Which organism is a third-order heterotroph? To what trophic level does that organism belong?



5. Which organism is an omnivore?



6. Which organisms belong to more than one food chain?



7. Which organism belongs to more than one trophic level?

Answers

1. 6

2. insect

3.plant

4.snake

5. snake

6. plant

7. bird

hope this helps, sorry if i got any wrong

Choose all the answers that apply.

Muscles in the small intestine are _____.

smooth
striated
involuntary
voluntary
cardiac
Pls pick multiple

Answers

Answer:

smooth and involuntary

Two layers of smooth muscle form the small intestine. The outermost layer is the thin, longitudinal muscle that contracts, relaxes, shortens, and lengthens the gut allowing food to move in one direction. The innermost layer is a thicker, circular muscle.

Also smooth muscle is not under our control so is called involuntary

No one ever helps me it would be nice if someone did , please give me the correct answer it’s easy :(

Answers

Answer: C

Explanation: bc the fertilizer is the toxic chemical

Answer:

it is most likely C, all the other choices seem redundant

Explanation:

How do i know if its entering or leaving or neither just by the precentage?

Answers

Answer:

1. Shrink

2. Swell

3. Neither

Explanation:

The percentage of water indicates how pure the water is, i.e. how concentrated the molecules of water are.

The higher the percentage of water, the more water molecules there are in the space.

Water molecules move from a high concentration to a low concentration across a semi permeable membrane, by the process of osmosis.

In beaker 1, the water outside is 89%, and inside the bag is 92%. That means the water inside the bag is at a higher concentration than outside. That means water will leave the bag, moving from a high concentration to a lower concentration outside. The bag will shrink.

In beaker 2, the water outside is 97%, and inside is 92£%. That means the water inside the bag is a lower concentration than outside. That means water will enter the bag, moving from a higher concentration outside to a lower concentration outside. The bag will swell.

In beaker 3, the concentration of water inside and outside is the same. That means they are in an equilibrium (the movement is balanced) so neither will occur

1. The least reactive elements on the periodic table are the _____________ (high number of valence electrons)

2. What makes a reactive metal reactive?

3. What makes a nonreactive noble gas nonreactive?
These are all the questions I need help with (plzzz help mee)

Answers

Explanation:

1. the noble gases because they have a full valence shell

2.the amount of valence electrons

3. they have a full valence shell and dont need any more electrons

What can be used to measure the rate of photosynthesis

Answers

Answer:

the answer it at the bottom

Explanation:

Uptake of CO2 by plants: Since CO2 is needed for photosynthesis, measuring how much of it is taken up by the plants gives us information on how much of photosynthesis is happening.

Release of O2: The amount of O2 produced during photosynthesis can be measured.

The percentage of a hunter-gatherer society’s diet provided by hunting versus gathering was mostly determined by _______.
a.
the climate
b.
the types of prey
c.
hunting skill
d.
the size of the human population

Answers

the answer is B. the types of prey

Answer:

a. the climate

Explanation:

Using f(x) = 3x + 4 find f(3)

Answers

Answer:

f(3)=13

Explanation:

f(x)=3x+4

f(3)=(3x3)+4

f(3)=9+4

f(3)=13

How could you test your hypothesis?
Find the total mass of the water and the dry
pasta, then cook the pasta and measure the
mass of the cooked pasta and the water in
which it is being cooked.
Find the mass of the dry pasta, then cook the
pasta, drain it, and find the mass of the
cooked pasta.

Answers

Answer:

Explanation:

Find the total mass of the water and the dry pasta, then cook the pasts and measure the mass of the cooked pasta and the water in which it is being cooked.

If plant has the same amount of sunlight but less water, then the process of glucose would A- increase, B-decrease, C- stay the same

Answers

A because glucose comes from sunlight and can be produced into glucose
The answer is a

Good luck

In 2019, what was the total income for all agriculture commodities?

Answers

Answer: Cash receipts for all commodities are forecast to decrease $12.3 billion (3.3 percent) to $358.3 billion (in nominal terms) in 2020. Total animal/ ...

Explanation:

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DNA Polymerase makes a copying error in a gene and an additional base
gets put into that gene. Which of the following is the most reasonable
result? *
1. The codons on the mRNA transcript contain an extra base which causes the amino
acid chain to not make the desired protein.
2. This causes RNA polymerase to transcribe the gene backwards.
3. The causes RNA polymerase to not recognize the gene and therefore no transcription
takes place.
4. The RNA polymerase recognizes extra bases and won't transcribe them.

Answers

Answer:

1. The codons on the mRNA transcript contain an extra base which causes the amino

acid chain to not make the desired protein.

Explanation:

Then error will make it not to produce the required output

pls like

one factor in the formation of hurricanes and tornadoes that is the same?

Answers

Perhaps the only similarity between tornadoes and hurricanes is that they both contain strong rotating winds that can cause damage. There are many differences between tornadoes and hurricanes. The largest tornado every observed was 4 km (2.5 mi) wide, and most tornadoes are < 0.8 km (0.5 mi) wide.

May mark me as brainlist please

How do scientists find the epicenter of an earthquake?

Answers

Scientists use triangulation to find the epicenter of an earthquake. When seismic data is collected from at least three different locations, it can be used to determine the epicenter by where it intersects.

Help please! thanks this due today!!!! :)

Answers

Answer:

Just shape the pigeon with cardboard than paint it!

Explanation:

Hope this helps have a great night!

Which statement describes the movement of water when a cell swells up and bursts due to osmosis?

Group of answer choices

The cell membrane used energy to move water into the cell through passive transport.

The cell membrane used energy to move water out of the cell through passive transport.

Water went out of the cell because the concentration of water inside the cell was higher than the concentration outside the cell.

Water went into the cell because the concentration of water inside the cell was lower than the concentration outside the cell.

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Question 9

Answers

Answer:

water went into the cell because the concentration of water inside the cell was lower than the concentration outside the cell.

Answer:

D. Water went into the cell because the concentration of water inside the cell was lower than the concentration outside the cell

please answer this for me

Answers

Answer:

Plants

Explanation:

In plant cells, the vacuoles are much larger than in animal cells

discuss the problems which farmers face who cultivate crops on hilly or mountainous areas

Answers

Answer:

The soil getting washed away

Explanation:

Is ATP used for long term storage?

Answers

Answer:

No

Explanation:

ATP is not used for long term storage.

ATP will release or give off energy when it releases a phosphate molecule. Since a cell can add or subtract a phosphate group, it has a way of storing and releasing energy. Most cells have only a small amount of ATP. It can't be used to store energy for long periods of time. These ATP stores last only a few seconds after which the breakdown of PC provides energy for another 5-8 seconds of activity. Combined, the ATP-PC system can sustain all-out exercise for up to 10-15 seconds and it is during this time that the potential rate for power output is at its greatest. These ATP stores last only a few seconds after which the breakdown of PC provides energy for another 5-8 seconds of activity. Combined, the ATP-PC system can sustain all-out exercise for up to 10-15 seconds and it is during this time that the potential rate for power output is at its greatest. Hence, ATP cannot be stored easily within cells, and the storage of carbon sources for ATP production (such as triglycerides or glycogen) is the best choice for energy maintenance. Lipids are molecules that can be used for long-term energy storage. Most cells have only a small amount of ATP. It can't be used to store energy for long periods of time. It is more efficient for a cell to keep a small supply of ATP. Cells can regenerate ATP from ADP as needed by using the energy in carbohydrates like glucose. The ATP molecule is just like a rechargeable battery. When it's fully charged, it's ATP. When it's run down, it's ADP. However, the battery doesn't get thrown away when it's run down–it just gets charged up again. Living organisms use two major types of energy storage. Energy-rich molecules such as glycogen and triglycerides store energy in the form of covalent chemical bonds. Cells synthesize such molecules and store them for later release of the energy. Monosaccharides
Monosaccharides contain just one sugar unit, so they're the smallest of the carbohydrates. (The prefix "mono-" means "one.") The small size of monosaccharides gives them a special role in digestion and metabolism. In a process called cellular respiration, chemical energy in food is converted into chemical energy that the cell can use, and stores it in molecules of ATP. ... When the cell needs energy to do work, ATP loses its 3rd phosphate group, releasing energy stored in the bond that the cell can use to do work.

Which of these organelles is responsible for protein synthesis?

Answers

Answer:

I would have to say either ribosomes or mitochondria.

Answer:

the ribosomes :)

Explanation:

What two parts of a nucleotide do not change throughout the structure of DNA?

Answers

Answer:

The second portion of the nucleotide is the sugar. Regardless of the nucleotide, the sugar is always the same. The difference is between DNA and RNA.

Explanation:

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Five-carbon sugar (deoxyribose sugar) and phosphate moiety are the two parts of a nucleotide which do not change throughout the structure of DNA.

What are Nucleotides?

Nucleotides are the organic molecules which are consisting of a nucleoside molecule and a phosphate moiety. They serve as the monomeric units of the nucleic acid polymers- deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), both of which are the essential biomolecules within all the different life-forms on the Earth.

Nucleosides are made up of nitrogenous bases and pentose sugar. There are changes at the nitrogenous bases in the different nucleoside molecules however, there are no changes in the pentose sugar and the phosphate moiety of the molecule.

Learn more about Nucleotides here:

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To become a virus, you only need 2 parts. One is Genetic Material or Genome (DNA or RNA) to
replicate it and the
or Protein Coat which protects the genome from digestive
enzymes. (Answer in lowercase) *

Answers

Answer: true

Explanation:

easy apes question help pls

Answers

Answer:D

Explanation:

The asteroid would have caused sever heat in the atmosphere and elinate most of the water so i is severe climate change

Answer:

D : The asteroid would have caused sever heat in the atmosphere and elinate most of the water so i is severe climate change

Explanation:

Large molecule formed when many smaller molecules bond together are called
metabolism
enzyme
solution
polymer

Answers

Answer:

The answer is Polymer

True or False: Enzymes have an optimal or ideal pH level?

Answers

Answer:

All enzymes have an ideal pH value, which is called optimal pH. Under the optimum pH conditions, each enzyme showed the maximum activity. For example, the optimum pH of an enzyme that works in the acidic environment of the human stomach is lower than that of an enzyme that works in a neutral environment of human blood. All enzymes have an optimum pH that they work best at. Its often neutral pH 7, but not always.

Explanation:

i hope this is good?

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Do you feel that our relatives past reflect our problems today, or do they reflect our Growth?at least one paragraph Which of these served as the government of the United States when the American revolution came to an end Which sentence contains a pronoun shift error? When we play football, our whole neighborhood joins in.If a singer wants to join the chorus, they have to audition.When writing of mine is read aloud, I get nervous. If his grades are high enough, he will get a scholarship NEED ANSWER ASAP PLEASE(Will get brainliest)Until 1989, most of Eastern Europe was part theEuropeanBritishSovietIndian I need help please... !!Which of the following statements best explains the relationship between medicines and concerns about the shrinking ofthe Amazon Rainforest?A. In an effort to protect the Amazon, the Brazilian government has limited the amount of medicalresearch that can be done in the rainforest.B. Creating medicine requires large amounts of resources, and harvesting these resources isleading to deforestation of the Amazon.C. The byproducts of many medicines are responsible for polluting the Amazon River.D. Many medicines are created from plants in the rainforest; without them, doctors and scientistsmay not be able to treat and cure many diseases. How were the social structures of Africa altered as a result of the forced migration of Africans destined for slavery in the Americas? PLEASE HURRY!!Look at the image below! How early colonist were able to get their houses built quick what would an equation be for 6 bags of flyers each bag containing 12 flyers A biker rides for h hours at a speed of s miles per hour and travels d miles. Which equation models the relationship? What is the solution to j + (-3) = 9? Which equation. Represents a line that is parralel to y = -2x-5 Reflect: You found that ( 3 + 4 x ) + ( 2 - x ) = 5 + 3xSuppose x=i (the imaginary unit)What equation do you get?Answer must include words/explanations (guys please help!!) Which of the following gives the area, A, of a circle with a diameter of 6 The sluggers go out for pizza after a game. Seven large pizzas plus two medium pizzas together have 100 slices. Five large pizzas plus six medium pizzas have 108 slices. How many slices are in each size? Which of these creates a dynamic story and propels the story forward ?a) the give and take between audience and artist b) the director concept c) the critic coming to the performance d)the respect of the artist for the audience what are the factors that influence management Explain the difference between representative and commodity money, and give examples of how each was used along the Silk Road. The 7th grade team went out for lunch. Their server brought the bill that totaled $72. The team wanted to tip the server 20% and there is also a 9% sales tax. How much will the 7th grade team spend after the tip and tax are added? Round your answer to the nearest cent if necessary. What job did 80 percent of people do in Egypt?