What is the difference between traditional PCR and qPCR?
a. Traditional PCR has only 1 cycle whereas qPCR has multiple cycles
b. In traditional PCR, the presence or absences of a DNA sequence is analyzed upon reaction completion whereas qPCR monitors DNA amplification as the reaction progresses
c. Traditional PCR is an outdated technique which is unreliable and has not been utilized for years whereas qPCR is a newer and better technique which is always preferred nowadays.
d. Traditional PCR is monitored by an increases in fluorescence signal whereas it is impossible to monitor qPCR by increases in fluorescence signal

Answers

Answer 1

Answer:

The difference between traditional PCR and qPCR (Real-time PCR) is:

b. In traditional PCR, the presence or absences of a DNA sequence is analyzed upon reaction completion whereas qPCR monitors DNA amplification as the reaction progresses

Explanation:

Real-time PCR (qPCR) is an advanced tool for analyzing RNA and DNA.  With Traditional PCR detection occurs at the end-point of the reaction, while with Real-time PCR, detection of the RNA and DNA sequencing is detected as the reaction is occurring.  The advantages of the qPCR over the traditional PCR include higher precision and resolution,  and increased dynamic range and sensitivity.

Polymerase Chain Reaction (PCR) is a widely-used Biology tool to rapidly replicate millions of copies of a specific DNA sample.


Related Questions

2. Name one positive benefit of having vertebrae for Chordates

Answers

It has some body equipment like ours. They have nerve chords, gill slits, segmented muscles, and a notochord; precursor of a backbone similar to the discs in human spines. Name one positive benefit of having vertebrae for Chordates. ... They can eat other animals and then increase their body size.


"What do the four genotypes inside the 4 boxes of a Punnett Square represent?

Answers

Answer:

They represent the percent chance to inherit a type of genotype. Recessive alleles, dominant alleles, heterozygous have chances to appear based off the parent alleles.

C cytosine need help with this problem

Answers

cytosine

Answer:

Explanation:

Question:
Using a specific example, discuss the importance of electrons in the transfer of energy in living systems.​

Answers

Answer: The transfer of energy in the form of electrons allows the cell to transfer and use energy in an incremental fashion. The principle electron carriers are NAD+ and NADH because they can be easily oxidized and reduced, respectively.

Explanation:

Electrons are very significant in the steps of energy transfer in the living body cells.

When the electrons are displaced from the molecule it gets oxidized which results in a reduction in the potential energy.

Electrons transfer is very important because most of the power is stored in the atoms in the form of electrons that are required for cell functioning.

The chief electron transporters are NADH and NAD⁺ they reduce and oxidizes the compounds.

NADis oxidized and NADH is the reduced form of the molecules.

The living structures cannot save a large amount of energy and hence they collect them in the form of ATP and electrons.

To learn more about the importance of electrons in living organisms follow the link:

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The amount of solar radiation received by the earth is greatest
at
a
The equator
b The mid-latitudes
С
The poles
d None of the above

Answers

The answer is A, the equator.

List advantages and disadvantages of using scientific models.

Answers

Answer:

Explanation:

disadvantages:

Models do not behave exactly like the things they represent. Accuracy—In order to make models simplistic enough to communicate ideas some accuracy is lost. For example, ball and stick models of atoms do not show all the details that scientists know about the structure of the atom.

advantages:

When students are engaged in scientific modeling, they are able to notice patterns and develop and revise representations that become useful models to predict and explain--making their own scientific knowledge stronger, helping them to think critically, and helping them know more about the nature of science.

The advantage of a scientific model is it allows complicated processes to be predicted and simplified. A model's drawback is that it could be deceptive and open to numerous interpretations.

What is a scientific model?

The advantages are:

Students who participate in scientific modeling are better able to identify patterns and create and revise representations that become useful models to predict and explain, strengthening their own scientific knowledge and fostering critical thinking. They also gain a better understanding of the nature of science.

The disadvantages are :

Models don't always act the same as the things they're meant to represent. Accuracy—Some accuracy is lost when models are simplified to the point where they can convey concepts. For instance, the specifics that scientists are aware of regarding the structure of the atom are not depicted in full by ball and stick representations of atoms.

Thus, the advantage and disadvantages are written above.

To learn more about the scientific model, refer to the link:

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describe the mass and charge of particles you find in the nucleus and in the electron cloud.

Answers

Proton is the positively charged particle with a charge equivalent to  1.6×10−19C .

Neutron is a neutral charged particle i.e. zero charge.

Electrons are negatively charged particle with a charge equivalent to  −1.6×10−19C .

Protons and Neutrons are inside the nucleus of an atom and contribute to the most of the mass of the atom. Electrons are revolving around the positively charged nucleus in quantized energy shells.

Sexual reproduction results in offspring that are *blank*
from both parents?
A. different
B. same
C. opposite
D. none of these

Answers

Answer:

I would put unique but since that's not a choice, probably different

Explanation:

Answer:

A. Different

Explanation:

In sexual reproduction, the offspring inherits traits from both parents. They are not an exact copy of either but have traits from both. Hope it helps :)

Scientists build models based on what they know from previous research to derive testable hypotheses. Independently, both Watson and Crick and their competitor Linus Pauling constructed an incorrect triple helix model with the nitrogenous bases arranged so they were on the exterior of the molecule and the phosphate groups on the interior. Although their model was wrong, what assumption made it reasonable to build a model with the bases projecting to the outside

Answers

Answer:

Nitrogenous bases contain the genetic information, their amount is variable among different species, and the arrangement of these bases is also variable among different species

Explanation:

Both Watson-Crick and Pauling's DNA models considered that DNA nitrogenous bases (i.e., Adenine, Cytosine, Thymine and Guanine) contain the genetic information that determines the characteristics of living organisms. Moreover, both DNA models also considered that nitrogenous base composition varies between species, as well as the arrangement of these bases in the DNA chain also varies between species. Based on these features, Linus Pauling considered that a model where nitrogenous bases would be arranged on the outside of the DNA molecule would be easier for the DNA molecule to be replicated, transcribed, or repaired. Although incorrect, Pauling's DNA triple helix model was fundamental to develop the helical (double-stranded) structure of DNA, which was finally discovered by Watson and Crick in 1953.

30 !!!
Around each electron that orbits an atom's nucleus ______.

A) a magnetic field

B) an electric current

C) a positive charge

D) a negative charge​

Answers

Answer:

the answer is D) a negative charge

Answer:

a negative charge

Explanation:

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6. Hawks have eyes that alow them to see prey from very far away. Which of the following
terms best describes this trait?
A vestigial organ
B. adaptation
Coverproduction
D artificial selection

Answers

The answer is B because eyesight is an example of a change or the process of change by which an organism or species becomes better suited to its environment.

11 Pause
Help
In the classification of organisms, which is the correct order of taxonomic groups from broadest to most specific?
A Order ----> Domain -> Genus
B. Class ---> Family -----> Order
Oc. Kingdom ----> Class -----> Phylum
D. Family ----> Genus ----> Species
Sion out
1:05

Answers

Answer:

I guess (D)

Explanation:

Linnaeus' hierarchical system of classification includes seven levels called taxa. They are, from largest to smallest, Kingdom, Phylum, Class, Order, Family, Genus, Species.

6.
Perform transcription by using the following piece of DNA.
Introns are underlined. Write the final strand of mRNA created.
TACCGIA CA ATG G GATC CouTube

Answers

Answer:

A-T-G-G-T G-T T-A-C C C-T-A-G

Explanation:

TACCGIA CA ATG G GATC

A-T-G-G-T G-T T-A-C C C-T-A-G

The "I" in the first few letters shouldn't probably be there since there are only 4 bases: A, T, C, and G.

Consider a population that contains black and white cats where the black allele (B) is dominant and the white allele (b) is recessive. Indicate whether each statement is true or false.
1. Evolution is occurring in a population of cats where the genotype frequencies of BB, Bb, and bb are 0.64, 0.32, and 0.04, respectively.
2. A population of cats is in Hardy-Weinberg equilibrium when the genotype frequencies of BB, Bb, bb are 0.49, 0.33, and 0.16, respectively.
3. Evolution is occurring in a population of cats where the genotype frequencies of BB and Bb are 0.7 and 0.2, respectively. _____
4. A population of cats is in Hardy-Weinberg equilibrium when the genotype frequencies of BB and Bb are 0.25 and 0.5, respectively. _____

Answers

Answer:

what is this what subject

Any mutation that affects just one base is called a -?- mutation.

Answers

Answer: A point mutation is when a single base pair is altered. Point mutations can have one of three effects. First, the base substitution can be a silent mutation where the altered codon corresponds to the same amino acid.

Explanation:

1. Which of the following events is not a source of genetic variation in eukaryotic organisms?

J. Mutation
K. Independent assortment
L. Mitosis
M. Fertilisation

2. Which statement best describes the process of endocytosis?

J. A vesicle within a cell fuses with the plasma membrane and releases its contents to the outside
K. Solid particles or liquid are taken up by a cell through invagination of the plasma membrane
L. Investment in one cytosis reduces the ability of the parent to assist another cytosis
M. One region of an embryo directs the development of neighbouring region of an embryo through movement of cells

3. In eukaryotic cells the conversion of ADP and phosphate into ATP occurs (KA1)

J. only in the mitochondria, and requires energy.
K. only in the cytoplasm, and releases energy.
L. in both the mitochondria and the cytoplasm, and requires energy.
M. in both the mitochondria and the cytoplasm, and releases energy.

4. One consequence of having small regulated steps in an energy pathway is (KA1)

J. an increase in the activation energy for the pathway.
K. a decrease in the amount of heat lost from the pathway.
L. a decrease in the amount of ATP released from the pathway.
M. an increase in the total energy released from the pathway.

5. Adult stem cells in bone marrow can differentiate into a variety of cell types e.g brain, skin, lungs, heart, liver and muscle.
Stem cells that are transplanted from a healthy donor into a patient (recipient) will divide and differentiate into cells of like form and function to the surrounding cells.
The adult stem cells from the bone marrow of the donor would be genetically identical to the cells of the

J. recipient, and divide by mitosis.
K. donor, and divide by mitosis.
L. recipient, and divide by meiosis.
M. donor, and divide by meiosis.

Answers

Answer:

1. Mitosis

2. K. Solid particles or liquid are taken up by a cell through invagination of the plasma membrane

3. L. in both the mitochondria and the cytoplasm, and requires energy.

4. J. an increase in the activation energy for the pathway.

5. K. donor, and divide by mitosis.

 

Explanation:

The sources of genetic variation in a population with sexual reproduction are 1-mutation, which can be defined as genetic changes in the genome of an organism that create new alleles in the population, 2-independent assortment, where pairs of homologous chromosomes are randomly separated, during meiosis, 3-fertilization, where two gametes held together to form the zygote cell (thereby creating new genetic combinations), and 4-recombination or crossing over (i.e., the exchange of genetic material between non-sister chromatids during meiosis).  Endocytosis is a cellular process by which substances enter into the cell by invagination of the cell membrane (this term is contrary to exocytosis). In eukaryotic cells, mitochondria are organelles whose membranes produce ATP from ADP and Pi by aerobic respiration (i.e., by the Krebs Cycle and electron transport chain), while glycolysis occurs in the cytoplasm. This process requires the addition of energy (chemical energy in the form of organic compounds). An energy pathway that has many small regulated steps is expected to produce intermediate products and increase its activation energy. Finally, stem cells that derive from a donor and are genetically identical it is expected that divide by mitosis.

which term best describes the role of a tick living on another animal?

Answers

Answer:

a) parasite

Explanation:

hopefully this helps

Answer:

A

Explanation:

Parasite, Because a tik living on another animal would be a "vampire" organism otherwise known as a parasite.

The ocean is the largest reservoir of carbon on Earth. Which of the following would most likely store carbon for the longest amount of time?
Calcium carbonate shells of marine organisms, like coral, oysters, and clams


Dissolved CO2 in surface ocean water


Dissolved CO2 in deep ocean water


Carbon stored in the rocks

Answers

Carbon is stored for the longest amount of time as Calcium carbonate shells of marine organisms, like coral, oysters, and clams.

What is the carbon cycle?

The carbon cycle is a cycle of how carbon is recycled between the atmosphere and the living and non-living part of the earth.

The carbon reservoir of the ocean forms part of the carbon cycle.

Carbon is stored in the ocean as calcium carbonate shells of marine organisms, like coral, oysters, and clams.

This is the longest time storage form of carbon in the ocean.

Learn more about carbon cycle at: https://brainly.com/question/25845923

What happens if the mass of the elements is greater compared to smaller ?

Answers

Hello. More information is needed to answer your question accurately. However, I will try to help you in the best possible way

If you are referring to what happens if the mass of an atom is greater compared to an atomic number, we can say that there is nothing too much, since it is normal for the atoms to have a mass slightly greater than the atomic number. The mass of an atom can never be less than the number of protons added to the number of neutrons. An atom with greater mass has a greater number of electrons and neutrons in relation to an atom of less mass and it is likely that the atom of less mass will donate or share electron with the atom of greater mass so that both reach the electric balance.

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a student made this model of carbon dioxide moleclue. it has one carbon atom and two oxygen atoms what is the correct way to write carbon dioxide the chemical name for
A: C2O
B: C²O
C: CO2
D: CO²

Answers

Answer:

CO₂

Explanation:

In chemistry we use subscripts after the element represented by that amount.

Answer:

C: CO2

Explanation:

Carbon has four valence electrons. Oxygen needs two valence electrons to become stable. A carbon dioxide molecule is a combination of one carbon atom and two oxygen atoms, CO2. Because carbon dioxide includes two different elements, it is a compound as well as a molecule.

Hope this helped!!!

How might a wildfire influence mass wasting? By drying out (removing the water) from the regolith on a slope, wildfires decrease the likelihood of a mass wasting event. A wildfire can dry out the regolith on a slope, making it less cohesive and more likely to travel downward. Wildfires burn only at the bottom of a slope, which oversteepens the slope and induces mass wasting. The ash from the wildfires lands on the slope, adding enough mass to instigate mass wasting. Because plants on a slope bind the regolith with their roots and shield the soil surface from raindrops, a wildfire that destroys these plants would render the soil looser and more susceptible to the erosional forces of rain.

Answers

Answer:

Because plants on a slope bind the regolith with their roots and shield the soil surface from raindrops, a wildfire that destroys these plants would render the soil looser and more susceptible to the erosional forces of rain.

Explanation:

The plants present in a region, protect the soil from being eroded by the impact of rain or wind. This is because the roots of the plants leave the soil more aggregated, block the impact of the wind and allow water to infiltrate. This protection is increased on slopes, where the chances of erosion are greater. However, when a fire occurs and these plants are killed, the roots no longer provide protection to the soil, leaving it drier and looser. This causes the wind to break this soil apart, facilitating erosion. In addition, rainwater has difficulties in infiltrating the soil and ends up draining, taking parts of the soil with it and causing erosion.

describe how the mammalian heart is adapted to it's function

Answers

Answer:

The ventricles are thick walled; to pump blood to the lungs and the rest of the body. The left ventricle has thicker wall to create more pressure because it pumps blood for longer distances round the body; while the right ventricle pumps blood to the lungs close to the heart.

Which of these would be an example of cultural bias in profiling?

a) Investigators are sure that their suspect is a tall male because they have found large footprints in the mud.
b) Investigators conclude that the victim allowed the suspect to enter the home, so they knew each other.
C) Investigators say that the suspect must be of a certain minority because that is who usually commits the crime in their precinct.
d) Investigators look for all the people leaving the crime scene to see if they are possible suspects.

Answers

Answer:

C) Investigators say that the suspect must be of a certain minority because that is who usually commits the crime in their precinct.

Explanation:

Cultural bias is the action where by someone holds a bias about a particular group of people or ethnicity due to the negative information which he or she had been feed with while growing up.

For example, when someone believes that, all crimes committed in America is done by African American people is a typical example of cultural bias. In the scenario given above, for the investigator to believe that, the suspect to be a certain minority rather than having an open view that it could have been anyone shows cultural bias and profiling of the investigators.

Which phase occurs immediately after a full moon?
waxing gibbous
waning crescent
waning gibbous
waxing crescent

Answers

waning gibbous

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which of the following are parts of the biosphere. choose all that apply

river
deer
tree
mountain
mushroom​

Answers

Mountain, tree, river

Plzzzzz help me. How does the number of eggs in one flower compare to the number of pollen grains in one anther?

Answers

A flower has more pollen grains than it does ovules so that the pollen has a better chance of getting to the ovules so they can make seeds so more plants will grow. Fertilization is when the sperm cell in the pollen joins with the egg cell so that a seed can form.

The table below shows the average distances of four planets from the sun.

Considering Kepler's Laws, which planet has the fastest average orbital speed?

A. Venus
B. Neptune
C. Saturn
D. Jupiter

Answers

Answer:

Venus

Explanation:

The closer a planet is to the Sun the faster it moves because the Sun can exert more gravitational force on it. Venus is the second closest planet to the Sun therefore the fastest out of the options.

Its Venus.

It is closer to the sun than the other planets on here.

All of the following are complications that can result from an individual suffering from an eating disorder except
A heart disease
B. dieting regularly
C. kidney damage
D. death

Answers

Answer:

The answer is B

Explanation:

Hope this helps

Answer:

B. dieting regulraly

Explanation: correct on edge 2021

just got it right ; )

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How was a round-the-world voyage useful to Darwin for developing his theory of evolution?

Answers

Answer: During the long voyage, Darwin made many observations that helped him form his theory of evolution. For example: He visited tropical rainforests and other new habitats where he saw many plants and animals he had never seen before (see Figure below). This impressed him with the great diversity of life.

Explanation:

Which type of macromolecules helps a cell brake down food? Lipids proteins carbonhydrates or nucliec acids

Answers

Answer:

The correct answer is - proteins.

Explanation:

All the food particles are broken down by specific protein molecules called enzymes. Carbohydrates are the macromolecules that are broken down by enzymes; amylase, lactase, sucrase, or maltase.

Proteins are macromolecules that are broken down with the help of the enzymes peptidase, pepsin. Lipids macromolecules are also broken down by lipase enzymes. Breakdown of these macromolecules provides energy for cellular activities.

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The figure below consists of a triangle, a rectangle, and a trapezoid. What is the area of the figure in square units? A. 39 B. 37 C. 49 D. 42 which of the following best describes a frameshift mutation. A. When a segment of nitrogen bases gets added into a strand of DNA. B. When a segment of nitrogen bases gets removed from a strand of DNA. C. When a segment of a chromosome gets removed. D. Both A and B. WILL MARK BRAINLIEST Which Chinese invention is still used for recording and transmitting the written word Help I screenshot help now!!!! How is religious life different in South Korea and China? John James Audubon published the volumes of Birds of America over an 11 year period, beginning in 1827.Audubon's Birds of America was popular because it reflected the era's - Which addition does the model below represent?++++OOO8+(-3)2+(-3)5+35+(-3) mehh need help withh this problem Can I get help for this?18. When you graph an inequality, you used a closed dot whenyou use which symbols?a) 5,2I b) >c),u d) >+19. When graphing an inequality, you use an open dot whenyou use which symbol?a) < >b) 3,2O c s,&d) 2, >20.What inequality does the number line graph represent?55132101 2 3 4 5 6a) x 54b) x2-4d) x A 41.6-kg person, running horizontally with a velocity of +4.21 m/s, jumps onto a 14.6-kg sled that is initially at rest. (a) Ignoring the effects of friction during the collision, find the velocity of the sled and person as they move away. (b) The sled and person coast 30.0 m on level snow before coming to rest. What is the coefficient of kinetic friction between the sled and the snow? 1. Write the word sentence as an equation. 2. Then solve the equation. 5/7a=25 easy algebra question Find the distance from A to C 1274^74 6 If triangle ABC is dilated using a scale factor of 2, what will be the coordinates of A after the transformation 7. Choose the correct way to write the underlined part of sentence 8. I will give brainleiest Was it just meFor the dabi simpswhen u realized u first liked him did u not admit it? What is a bright streak of light that results from a space rock burning in earths atmosphere? A. Meteor B. Asteroid C. Meteorite D. Meteoroid Find the mean, median, and mode of the following data set 17, 18, 13, 15, 12, 11, 35, 18, 14 Sierra is writing an email to her city councilman suggesting that the citys bicycle rental program be extended to her neighborhood. After receiving some feedback from her mother, she knows she needs to make some changes to make her writing more effective. Read the following paragraph from her first draft.(1) Our neighborhood needs the citys bicycle rental program. (2) Public transportation doesnt reach us, so many of us have to walk everywhere. (3) In addition, if they could rent bikes, people with cars could avoid having to drive into the city. (4) Everyone in this neighborhood says this would help us out a lot.Which sentence does Sierra need to rewrite in order to further develop and strengthen her argument? A. sentence 1 B. sentence 2 C. sentence 3 D. sentence 4