Design an experiment to test the role of thyroxine in helping the body adjust to cold temperatures. Describe the overall investigation to be done, the variables to be changed, the variables to be kept constant, the subjects to be used, the control and experimental conditions to be used, the type of data collected, and how the data will be collected. Explain how these data can be used to justify the conclusion that thyroxine is involved in adjusting body temperature in response to cold. (9 points)

Answers

Answer 1

Answer:

- Hypothesis: thyroxine gene is responsive to low temperatures, thereby its transcript level is higher in cold climates

- Subjects of study: gene expression data collected from individuals from countries with different climates  

- Experiment: to determine thyroxin gene expression level by Real-Time PCR (RT-PCR) in individuals living in cold climates (e.g., Iceland) versus individuals living in warmer climates (e.g., Puerto Rico)

- Independent variable: temperature

- Dependent variable: thyroxin transcript level  

- N sample: 500 individuals of each population  

- Control group: individuals living in the same climate conditions but from different countries. It can be done by collecting gene expression data from individuals living in another country. For example, both Fairbanks (Alaska) in the USA and Reykjavik in Iceland are at 64 degrees N latitude, and thereby these cities have similar climates

- Constant variable: all data collected from individuals of adult age (age range: 20-30 years)  

Explanation:

The scientific method is a sequential process that consists of making hypotheses in order to explain observations from the real world, make predictions based on the working hypotheses, carrying out experiments based on those predictions, test the predictions and draw conclusions based on the results obtained. In an experiment, the independent variable isn't changed by the other variables, while the dependent variable is the variable being measured in the experiment. A constant variable does not change during the experiment. Moreover, a control group is a particular group to which comparisons are made during the experiment (the control group isn't exposed to the independent variable, and therefore any change in the experimental groups can be compared with the control). In this case, it is expected to measure thyroxine expression levels in 500 individuals from each country by RT-PCR (RT-PCR technique is used to measure the amount of mRNA of a target gene of interest). Subsequently, it is possible to determine if the differences in the thyroxin gene expression levels between populations (i.e., individuals living in Iceland vs individuals living in Puerto Rico) are statistically significant to confirm that thyroxin gene activity is increased in response to cold exposure. Finally, the control group can be used to determine if observed differences in gene expression are irrefutably correlated with cold conditions.


Related Questions

what is angiosperm in plant

Answers

Answer: a plant that has flowers and produces seeds enclosed within a carpel. The angiosperms are a large group and include herbaceous plants, shrubs, grasses, and most trees.

Explanation:

Why can't weeds take over the world? In other words, what limits the growth of populations?

Answers

Answer and Explanation:

Many elements can limit population growth. The main elements that can cause this are the lack of resources necessary for the life and development of living beings and the lack of space to be taken by them. In relation to weeds, these resources can be the availability of sun, amount of soil, soil fertility, availability of water and space. However, these plants are very resistant to the lack of these resources, and what most limits their growth is human interference.

Weeds are unfavorable to agriculture and other human activities. This makes humans fight the population growth of these plants using chemical products and even physical strategies. This interference prevents weeds from taking over the world.

Please help I’ll mark you as brainliest if correct! #2

Answers

Answer:

Tt

Explanation:

Hello. I believe that you are only referring to question 2, since question 3 does not present all the answer options. As such, the offspring of a cross between a tall pea plant (TT) and a low pea plant (tt) would be completely "Tt."

To achieve this result, you must make a Punnett Square, which is a frame that allows you to combine the alleles of the parents in a cross to project the alleles of the offspring.

Alleles are those characterized by the letters associated with characteristics. As you saw in the question above, tall pea plants have "TT" alleles that are dominant alleles, while low pea plants have "tt" alleles that are recessive alleles. In this case, to use Punnett Square, you must put the alleles of one parent in the first row and the allele of the other parent in the first column. Then just combine each allele, as shown below:

    T    T

t    Tt   Tt

t    Tt   Tt

As you can see all the offspring will have the "Tt" alleles. This means that the entire offspring will be tall pea plants. This is because the recessive allele "t", which is related to pea plants, can only be expressed in the presence of another recessive allele. However, the dominant "T" allele, which is associated with tall pea plants, can be expressed in any situation.

Which of the following is true about the coasts of North and South America? O A. Little industry is located along the coasts, B. There are few cities and the population is sparse. O c. A majority of the population is located there. D. People go to the coasts only when on vacation,​

Answers

Answer:

The correct answer is C. In North and South America, a majority of the population is located on the coasts.

Explanation:

Historically, the vast majority of civilizations have sought to establish their urban centers around bodies of water, be they rivers or seas, that allow them a rapid expansion outward as well as channels through which to channel trade. Thus, it can be seen how throughout the world and especially the American continent, large cities and population centers are located primarily on the coasts. Examples of this situation are Vancouver in Canada, New York and Los Angeles in the United States, Rio de Janeiro in Brazil or Buenos Aires in Argentina.

9. If the Earth and the Moon were on the same orbital plane, how often would a lunar eclipse
occur?
A. Once a month at the First Quarter phase.
B. Once a month at the full Moon phase.
C. Once a month at the New Moon phase.
D. Once a month at the Third Quarter phase.

Answers

Answer:

.B

Explanation:

once at the full moon phase

Why is global warning considered a controversial issue?

Answers

answer: The global warming controversy concerns the public debate over whether global warming is occurring, how much has occurred in modern times, what has caused it, what its effects will be, whether any action can or should be taken to curb it, and if so what that action should be. In the scientific literature, there is a strong consensus that global surface temperatures have increased in recent decades and that the trend is caused by human-induced emissions of greenhouse gases.[1][2][3][4][5][6] No scientific body of national or international standing disagrees with this view,[7] though a few organizations with members in extractive industries hold non-committal positions,[8] and some have attempted to convince the public that climate change is not happening, or if the climate is changing it is not because of human influence,[9] attempting to sow doubt in the scientific consensus.

Phenylketonuria causes a severe cognitive disability due to the inability to metabolize phenylalanine. It is an autosomal recessive disorder. About one in 12,000 newborns in Massachusetts are afflicted with the disease, skewed higher than the general US population due to more Irish-Americans. Assuming the gene is in Hardy-Weinberg equilibrium, the frequency of the carriers (heterozygotes) in Massachusetts would be calculated to be...

Answers

Answer:

The frequency of the carriers (heterozygotes) in Massachusetts is 0.018

Explanation:

Due to technical problems, you will find the complete explanation in the attached files

Please answer quickly

Answers

22. Species change over time
23. Observing

What hormones stimulate the cycle of egg or sperm production?

Answers

Answer: The main reproductive hormones are estrogen and testosterone. Once a girl enters puberty, estrogen matures the eggs in the ovaries. These are then released at regular intervals during the menstrual cycle. Testosterone stimulates the production of sperm in men.

Explanation:

Changes in the plant species in an area cause changes to populations of animal species in the area too. Propose a reason why this occurs.



Give a simple answer please ​

Answers

Because animals need food dumas

I’m struggling with this practice problem help

Answers

Answer:

it think it C ^^^^^^^^^^^^

Can you guys help me

Answers

survival of the fittest

Imagine two populations of penguins live in the same ecosystem. One population begins to exchange stones as a mating ritual while the other population begins to dance by shifting from one foot to the other. Individuals from each population will only mate with individuals that display the appropriate mating ritual. Which type of reproductive isolation mechanism does this scenario describe?(1 point)

Behavioral Isolation

Geographic Isolation

Temporal Isolation

Habitat Isolation

Answers

Answer:

i think its behavioral

Explanation:

Answer:

The answer would be Behavioral Isolation.

Explanation:

I did the Connections Academy Quick Check and it said it was correct.

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