A protein isolated from a thermophilic bacterium shows a molecular weight of 160 kD when eluted from a size-exclusion chromatography column, but when the purified protein is run on an SDS-PAGE gel, a single band of 80 kD is observed. These results tell us that the protein is eluting from the size-exclusion column as a

Answers

Answer 1

Answer:

as a dimer consisting of two identical monomers (80 kDa subunits) that are packed together via hydrophobic interactions

Explanation:

SDS-PAGE (sodium dodecyl sulphate–polyacrylamide gel electrophoresis), is an electrophoretic methodology used to separate proteins that have a molecular weight between 5 to 250 kDa. SDS is a well-known ionic detergent that is able to break hydrophobic interactions and hydrogen bonds. Moreover, size-exclusion chromatography is a filtration technique that separates molecules in solution according to their molecular size. In this case, SDS-PAGE showed that the target protein is composed of two identical subunits (monomers) of 80 kDa each, which were separated by the detergent and formed one single band in the SDS-PAGE gel.


Related Questions

1. Which of the following has the smallest effect on the rate of photosynthesis?


A. Light intensity

B. Temperature

C. Oxygen concentration

D. Carbon Dioxide



2. The tiny mouth wide openings in leaves where gases move in and out is called ?

A. Stroma

B. Stromata

C. Sweat Pore

D. Mesophyll

Answers

Answer:

D: Carbon Dioxide

B: Stromata

All organs in the human body perform important functions. Which organ controls all
of the other organs and is called the control center of the body?
stomach
B
small intestine
С
brain
D
liver

Answers

Answer:

c

Explanation:

Answer:

Well I thought it was the nervous system but that is connected/ made up of the brain so I would go for C, Brain

which organelles are important to the life process of metabolism

Answers

Answer:

Cellular Metabolism

Explanation:

Im pretty sure thats it!

Answer:

the mitochondrion and the chloroplast.

Explanation:

The following can be very useful to plants in nitrogen cycle except ______________
a)nitrifying bacteria
b)denetrifying bacteria
c)lightening
d) nitrogen fixing bacteria

Answers

C) Lightening
Nitrogen cycle can not exist without bacteria

Which of the following statements about the cells of any multicellular organism is true? More than one answer may be correct.

Answers

Answer:

C.) (Different genes are expressed in different types of sells.)

Explanation:

I hope its help;)(;

This question may be completed independently or as a group exercise. You have learned that humans have 46 total chromosomes, and chimpanzees have 48 total chromosomes. Use other textbooks in biological anthropology and biology (or reputable online resources) to find the number of chromosomes in other organisms. Give an organism with considerably more chromosomes than humans. Give an organism with considerably fewer chromosomes than humans. Did your findings surprise you

Answers

Answer:

In comparison to humans, there are more chromosomes in the Hermit crab that is 127 pairs of chromosomes for a grand total of 254 and in Myrmecia pilosula has fewer chromosomes than humans.

The complexity of an organism or how advance is an organism has nothing to do with the number of chromosomes it had. The chromosome number is subject to change over time. Human ancestors had more chromosome numbers as compared to now but they are not advanced to us.

The bark of a tree trunk includes which of the following tissues?
A. primary and secondary phloem
B. Heartwood and phloem
C. Heartwood and sapwood
D. Secondary phloem and layers of periderm

Answers

D. Secondary online and layers of periderm


In what area of the world does the low pressure area stay in the winter? Why?

Answers

Answer:

Atlantic because minuses 0 degrees

Members of the Kingdom Plantae are characterized by _ while members of the Kingdom Fungi are characterized by ---

cell walls of cellulose; cell walls of chitin

heterotrophy, autotrophy

mostly unicellular organisms; having segmented bodies with jointed appendages

having segmented bodies with jointed appendages; mostly unicellular organisms
d

Answers

I am pretty sure it is A

Answer:

Heterotrophy, Autotrophy

Reasoning: Heterotrophy organisms are organisms who can't retrieve food themselves and use energy and sunlight in order to get their food, which is exactly what plant cells do. Autotrophy is the ability to produce things such as carbon dioxide and oxygen, which plants do as well. Hence, Heterotrophy and Autotrophy are the most reasonable answers.

PLEASE HELP ASAP! WILL GIVE BRAINLIEST TO CORRECT ANSWER! HELP!! HELP!!
The diagram shows the structure of an animal cell.



The image of an animal cell is shown with some organelles labeled numerically from 1 to 6. The outer double layer boundary of the cell is labeled 1. A stacked disc like structure is labeled 2. A broad rod shaped structure with an irregular shape inside it is labeled 3. The entire plain section that forms the background of the cell and is within the outer boundary is labeled 4. A small circular shape within the large circular shape is labeled 5. The large central circular shape is labeled 6.


Which number label represents the cell membrane?


1

2

4

6

(this is middle school science)

Answers

Answer:

4

Explanation:

Answer:

4

Explanation:

I took the test

Emily's dog has puppies. 3 puppies are brown and 2 puppies have spots. The difference in their coat color is known as A trait B feature r variation​

Answers

Answer:

What

Explanation:

Answer:

This is known as A. Trait

the movement of rock by wind is known as _____

can someone help me with this?

Answers

Answer: erosion?

Explanation: the definition of erosion is the movement of rock particles by wind.

Answer: i agree with the person above me

Explanation: it's erosion

The endocrine system and the nervous system both send messages that control processes in other parts of the body. Which is an example of a process caused mainly by the endocrine system?
A. developing allergies to pollen, pet hair, or specific foods
B. regulating growth and functions of certain body organs
C. improving your skills at a sport or other physical activity
D.pulling your hand away when you touch a hot stove

Which picture above accurately shows an example of the circulatory organ system in a zebra?
A. figure 2
B. figure 3
C. figure 4
D. figure 5

Answers

B. They regulate growth and function of certain body organs. This system releases hormone and sends signals to the body for when to grow, or when you need to drink and releases adrenaline.

Figure 3. A heart is a main part of the circulatory system.

can someone explain what's going on in the graph?

Answers

So as you see it’s going up on the x axis
in the graph it’s going up in the x axis

Which energy source provides most of the world's energy consumption?
natural gas
hydroelectric
solar
fossil fuels

Answers

Answer:

The energy source which provides most of the world's energy consumption is

Explanation:

Ans = Hydroelectric

true or false:
One difference between somatic cells and gametes is that somatic cells are identical to one another, while gametes are not

Answers

Answer:

true.

Explanation:

all the somatic cells of an organism have same number of chromosomes while gametes are haploid and have half number of chromosomes. so gametes are different from somatic cells.

Where does competition for reproduction mostly likely occur

Answers

Finding a mate??? I’m not sure
It's population because a community is the number of species able to reproduce in an area.

Oil is a nonrenewable resource because it:

is made from plant remains
will never run out
takes millions of years to make
is quickly and easily made

Answers

Answer:

C. Takes millions of years to make

Choose the word that best completes each sentence. Angiosperms’ anchor them in the ground and absorb water for the plant. Their stems provide support to the plant and connect the roots and leaves. Their capture the sun’s energy and use it to make food through the process of photosynthesis. Angiosperms’ contain the reproductive parts of the plant. Their protect and help spread the seeds.

Answers

Answer:

Root anchor them in the ground and absorb water for the plant. Woody stem provides support to the plant and connect the roots and leaves. Leaves capture the sun's energy and use it to make food through the process of photosynthesis. Flowers contain the reproductive parts of the plant. fruite protect and help spread the seeds.

Explanation:

Answer:

roots, leaves, flowers, fruits

Explanation:

Can’t roots be eaten as food? True or false.

Answers

Answer:

false

Explanation:

hope dis helps

Answer:

true, people have been eating roots for years

Hope this helps!!!

plz mark brainliest

Explanation:

The volume of a liquid is often given in a. meters. c. liters. b. centimeters. d. square units. Please select the best answer from the choices provided A B C D Mark this and return

Answers

Answer:

C

Explanation:

Liters is a normally used measurement for liquids.

Match the words to the definitions

Answers

Sexual Reproduction
Zygote
Fertilization
Chromosomes
Sexual reproduction
Zygote
Fertilization
Chromosomes

Which of the following dietary fats is considered to be the least healthy?

Trans unsaturated fat
All dietary fats are unhealthy
Cholesterol
Saturated fat

Answers

Trans unsaturated fat.

Fats that increases the bad cholesterol in the body are considered worst. Thus, Trans unsaturated fat is the correct answer.

What are trans fat?

Unsaturated fatty acids have the presence of double bonds. This double bond is either 'cis' or 'trans' in origin.

When the unsaturated compounds are linked through trans bond, they form trans fat.

Trans fat are produced through chemical reaction. This reaction is termed as partial hydrogenation.

Trans fats have shown potential damage to the human body in research studies. Trans fat raises the bad cholesterol level (more lipid, less protein) in the body.

This can lead to several heart disease, liver damage or even atherosclerosis. Such diseases can be life threatening.

Trans fat are found mainly in fried and processed food. The junk food also contains high levels of trans fat.

Therefore, consumption of trans fat should be avoided for health safety.

Learn more about trans fat, here:

https://brainly.com/question/10509593

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There are 252 deer in a population. There is no net immigration or emigration. If 47 deer die and 32 deer are born in one month, what is the population size at the end of the month? Round your answer to the nearest whole number

Answers

Answer:

240

Explanation:

252-47=205

205+32=237

237 to the nearest whole number is 240

hope it helps

Which of the following is another way to refer to body cells?

a. sex cells

b. gametes

c. mitosis

d. somatic cells

Answers

Answer:

: somatic cell —opposed to germ cell. 2 : the one of two cells produced by division of the generative cell in the pollen grain of certain gymnosperms that in turn divides to produce two male nuclei or cells

Answer:

D is the answer I'm pretty sure :)

4. Is there a relationship between the number of black boxes and any of the water pumps. Be specific and detailed in your answer. .​

Answers

Answer: Cholerae is fecal contamination of water, meaning people can only get this by using or drinking the contaminated water. If a water pump has the contaminated water, boxes near that water pump will seem higher than areas with clean water.

Explanation:

A plant and an animal are both living things. According to the Cell Theory, what can you conclude about these two
very different organisms? SC.912.L.14.1
Plants have cells but animals do not
They are both made of one or more cells.
They both come from the same kind of cell.
O They both come from a non-living organism.

Answers

Answer:

They are both made of one or more cells.

Explanation:

A plant and an animal are both living things. According to the Cell Theory, what we can conclude about these two very different organisms is that they are both made of one or more cells.

A cell can be defined as the fundamental or basic functional, structural and smallest unit of life for all living organisms. Some living organisms are unicellular while others are multicellular in nature.

A unicellular organism refers to a living organism that possess a single-cell while a multicellular organism has many (multiple) cells.

Generally, cells have the ability to independently replicate themselves.

In a cell, the "workers" that perform various functions or tasks for the survival of the living organism are referred to as organelles and they include nucleus, cytoplasm, cell membrane, golgi apparatus, mitochondria, lysosomes, ribosomes, chromosomes, endoplasmic reticulum, vesicles, etc.

What is the world's smallest country?

Answers

Vatican City is the smallest country in the world

Answer:

Vatican City is the smallest country in the world, with an area of 526,235.6 square yards.

Explanation:

NEED HELP WITH THIS QUESTION!!! PLEASE HELP ME!!!

Answers

Answer: xylem

Explanation:

Direction of transport : Xylem= Upwards

Phloem=Upwards and downwards

Diagrams 1 and 2 show cells from an organism with a diploid chromosome number of 4.
> >
x x
1
2
Refer to the illustration above. Which of the cells will be a diploid cell at the completion
of division?

Answers

Yes it is easy my guy it’s b just wait til u get to chemistry

Both the cells will be diploid cells at the completion of division. Therefore, the correct option is B.

In diagram 1, the chromosomes are in the metaphase of cell division, that is aligned at the centre of the cell. In the next phase which is anaphase, these chromosomes are pulled apart by the spindle fibres in opposite directions. As a result, the chromosomes are split into half and each cell gets half the number of chromosomes in the form of chromatids. Thus, each cell will have 4 chromatids.

In diagram 2, the chromosomes are in the early anaphase stage. When the cell will split into two, each daughter cell will have 2 chromosomes. These chromosomes separate in the telophase and thus, each cell will have 4 chromatids.

Learn more about cell division in:

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