A 1.0-kb DNA fragment from the 5' end of the mouse gene is examined by DNA footprint protection analysis. Two samples are end-labeled with 32P, and one of the two is mixed with TFIIB, TFIID, and RNA polymerase II. Both samples are exposed to DNase I. The results are displayed below. The DNA exposed to proteins is run in the right-hand lane of the gel. Control DNA is in the left-hand lane.

Required:
What length of DNA is bound by the transcriptional proteins?

Answers

Answer 1

Answer:

Explanation:

Proteins, such as RNA (ribonucleic acid) polymerase, were loaded into lane 2. TFIIB and TFIID are two forms of TFII. These proteins attach to the fragment of DNA. Furthermore, these proteins shield DNA from the DNase enzyme, which may trigger DNA to split. As a result of the above findings, we may conclude that the inserting proteins(RNA polymerase, TFIID and TR 8 proteins) cover the area between 500 and 800 bp (base pair) in the DNase I footprinting experiment.

As a consequence, the length duration is 300bp since the promoter area is about 500-800bp and is not digested by DNase after incubation of inserting proteins.

A 1.0-kb DNA Fragment From The 5' End Of The Mouse Gene Is Examined By DNA Footprint Protection Analysis.

Related Questions

!!FOR PLANT SYSTEMS!!
Given the loss of farming jobs projected in the near future, distinguish at least two factors in a decision one might make to enter the field.

Answers

i cant process this lol no matter how much tiems read it

Answer:

this is the suggested answer

Explanation:

A correct answer will discuss two factors from among the following: one factor is despite the projected loss in farming jobs, these jobs are still essential and may still provide for a lucrative and successful career given that the median income level that is significantly higher than the national average. Another factor is the wide variety of careers in this general field, so the information on declining career outlook could be misleading, depending what specific career one is looking into. Another factor is the several training programs available to benefit those looking to enter this field, which can contribute to success.

Ashton is a crime scene investigator. He ensures that all the evidence gathered at the scene is properly handled and labeled. What is Ashton protecting?
Ashton ensures that the (blank) the documentation of who possesses evidence at all times, is protected by properly handling and labeling evidence.

Answers

when it says (blank) it means evidence evidence.

Which of the following is an example of erosion?

Answers

Answer:

A

Explanation:

I think A is the example of erosion

Which type of plant lives the longest?
a.annual
b.biennial
c.perennial
d.none of the above

Answers

Answer:

c. perennial

Explanation:

A silver maple oak tree is an example of a perennial as it can live hundreds of years

Signal Sequences: 1)A signal sequence is made of: a.Nucleotides b.Amino acids c.Lipids d.Carbohydrates 2)All protein synthesis starts on ribosomes that are located in the _________________. 3)If a molecule lacks a signal sequence it will end up in the ______________ 4)Proteins with a nuclear localization sequence (NLS) are generated on ribosomes located in the ___________________. 5)Proteins with a mitochondrial signal sequence are generated in ribosomes located in the _________________.

Answers

Answer:

1. Option B

2. cytoplasm

3. cytosol

4.  nucleus

5. mitochondria

Explanation:

1. Option B

A signal sequence consists of hydrophobic  amino acids .

2. cytoplasm in prokaryotes

3. cytosol

4.  nucleus

5. mitochondria

The following statements refer to a comparison of reproduction in ferns vs. flowering plants. Indicate whether each statement is true or false by writing true or false in each blank. true Both ferns and flowering plants produce spores at some point in their life cycles. false Only ferns have a gametophyte as part of their life cycle. Only flowering plants produce pollen grains. true Both a tree and a large fern plant are diploid sporophytes.

Answers

Answer:

Both ferns and flowering plants produce spores at some point in their life cycles. True

Only ferns have a gametophyte as part of their life cycle. False.

Only flowering plants produce pollen grains. true

Both a tree and a large fern plant are diploid sporophytes. True

Explanation:

Yes, both ferns and flowering plants produce spores in their life cycles. In fern plant, it produce spores on the underside of the leaves whereas in flowering plant, there are two types of spores such as microspores and megaspores. Both ferns and flowering plants have gametophyte as part of their life cycle. Flowering plants produce pollen grains whereas non-flowering plants produce spores to continue their generation. Both tree and large fern plants having diploid sporophytes which is a necessary part of their life cycle.

A bacteria structure involved in motility.

Answers

Answer:

flagella

Explanation:

Thin long thread like structure that help in locomotion in bacteria.

Different alleles produce different characteristics. True False

Answers

Answer:

Explanation:

Los alelos son formas alternas de un gen, que difieren en secuencia o función. Toda característica genéticamente determinada depende de la acción de cuando menos un par de genes homólogos, que se denominan alelos.

Friction reduces efficiency because energy is lost as ____.
a.
work
c.
gravity
b.
heat
d.
thrust


Please select the best answer from the choices provided

A
B
C
D

Answers

Answer:

option (b) heat is right answer

24. all of the following are function of sER Except ?

A. Synthesis of lipid

B. Carbohydrate Metabolism

C. Detoxification of drug and poisons

D. Modification of protein​

Answers

Answer:

D

Explanation:

The organelle that modifies proteins is the golgi body.

D
It’s d I hav done it before

Why are lipids and proteins free to move laterally in membranes?
A) The interior of the membrane is filled with liquid water.
B) There are no covalent bonds between lipid and protein in the membrane.
C) Hydrophilic portions of the lipids are in the interior of the membrane.
D) There are only weak hydrophobic interactions in the interior of the membrane.
E) Molecules such as cellulose can pull them in various directions.

Answers

D. There are only a week Hydrophobic interactions in the interior of the membrane.

what is the answer? for this

Answers

Answer:

b

Explanation:

because it is the least inclined

If an organism has a diploid number of 54, how many chromosomes will be in each of its gametes?
a. 54
b. 52
c. 27
d. 104
e. 108

Answers

The answer to this question is D

There will be 27 chromosomes in each of its gametes.

Meiosis is also called reductional division, in the process s-ex cells, that is, sperm and egg are produced, and each cell has the half number of chromosomes than its parent cells.

• In the given case, the organism is diploid with 54 chromosomes, that is, 2n = 54.

• Here n is the haploid number of chromosomes, in the given case, n = 27, that is, the haploid number of chromosomes is 27.

• Therefore, the sperm and egg cells have 27 number of chromosomes each.

Thus, the correct answer is option c, that is, 27.

To know more about:

https://brainly.com/question/23874505

What is a population bottleneck?

Answers

Answer:

A population bottleneck or genetic bottleneck is a sharp reduction in the size of a population due to environmental events such as famines, earthquakes, floods, fires, disease, and droughts or human activities such as specicide and human population planning.

Explanation:

Explanation:

A population bottleneck or genetic bottleneck is a sharp reduction in the size of a population due to environmental events such as famines, earthquakes, floods, fires, disease, and droughts or human activities such as specicide and human population planning.follow me

____________ are made of a series of paired statements that describe physical characteristics of different organisms.

Answers

Answer:

taxonomic key

this is A series of paired statements that describe the physical characteristics of differnent organisms.

Answer:

taxonomic key

Explanation:

A series of paired statements that describe the physical characteristics of differnent organisms.

DNA sequence that codes for glycine​

Answers

qwertyuiopal!!!! :))))

Help asap plsss no link or websites I think brainley has a bot problem

An airplane flies with a constant speed of 760 km/h. How far can it travel in 2 1/2 hours? Distance = ​

Answers

Answer:

I would say 1520 miles is your answer

Which of the following is an example of the endocrine system maintaining homeostasis? (3 points)

-Detecting a pain stimulus and sending a signal to the spinal cord to perform an automatic response
-Discharging an excessive amount of hormones in the blood and not sending a signal to stop production
-Sending a message to the pituitary gland to start producing a hormone when the levels in the body are too low
-Using sense organs to get information about the outside world and direct an appropriate body response release

Answers

Answer:

-Discharging an excessive amount of hormones in the blood and not sending a signal to stop production

Explanation:

Endocrine system is regulated by negative feedback mechanisms that work to maintain homeostasis.

Concentration of hormones, and how they effect other body systems, are controlled in this manner.

For instance, the blood glucose regulation is controlled by insulin and glucagon, hormones produced by the pancreas.

A farmer grows wheat near a river. He frequently uses
fertilizer on his fields, and when he waters the fields,
excess water runs off into the river.
What should the farmer be most careful to monitor to determine his farm's
impact on water quality?
A. Type of fish in the river
B. Amount of water in the farm's soil
C. pH of rainwater falling on the farm
D. Level of nitrates in runoff water

Answers

Answer: I think its B

Explanation: I hope this helps :)

Answer:

D. Level of nitrates in runoff water

Explanation:

Took the test too :)

Scientists analyzed data collected over a period of years in a game reserve in South Africa to test the hypothesis that competition with lions limits the abundance of leopards in the same community. The game reserve encompasses a variety of habitats, from open grassland to wooded areas around rivers and streams. It contains most of the indigenous mammal species and an adequate availability of their prey. For the most part, the leopards and lions show similar habitat preferences in the reserve.

The mass of the average lion is about three times that of the average leopard. The scientists analyzed several factors that might influence the abundance of each population, including the sizes of the animals preyed on by each species

Size of Prey Animal Percent of Leopards’ Prey (±2SEx¯) Percent of Lions’ Prey (±2SEx¯)
Small 34 ± 2 3 ± 1
Medium 63 ± 3 23 ± 2
Large 3 ± 1 36 ± 4
Very large 0 38 ± 3

Because competition with lions might affect the population size of the leopards, the scientists also analyzed data on the lion and leopard population sizes in the game reserve (Figure 1).

Required:
Describe what scientists mean when they refer to an ecological community such as that shared by the leopards and lions.

Answers

Answer:

The correct answer would be - these are the ecological community that shows the interspecific competition.

Explanation:

In this ecological community, both predators lion and leopards are living together and by the given data and information it is evident that they both share the same habitat and they both depend on the same prey for their food.

This cause competition between both lion and leopard for food and habitat. Such competition or interaction is referred to as the interspecific competitions negatively impacted by the presence of the other because they will have less food.

Answer

An ecological community refers to all of the interacting populations in a defined habitat.

Explanation:

see above

From the list of activities below, select those that can increase your carbon footprint.

installing solar panels on your home
driving a car that uses gasoline
keeping non-LED lights on in your home all night
purchasing foods processed in a factory
planting a garden

Answers

Answer:

driving a car the uses gasoline

keeping non-led lights on in your home all night

purchasing foods processed in a factory

Answer:

driving a car that uses gasoline

keeping non-LED lights on in your home all night

purchasing foods processed in a factory

Explanation:

Hydrogen bonds are formed as a result of which of the following? A. Ionic bonds b. Nonpolar covalent bonds c. Polar covalent bonds d. None of these are correct

Answers

Answer:

C

Explanation:

I believe they're opposite.

The moon's relative motion causes
A)
ocean tides.
B)
wind currents.
the phases of the Moon.
D)
the rotation of the Moon.

Answers

Answer: the phases of the moon

Explanation: i did it

A solid is that form of matter that possesses the following
characteristics___.
a Particles vibrate around fixed axes
b Definite shape
C Definite volume
d All of the above

Answers

all of the above lol.

critically discuss the transport mechanisms across the membrane​

Answers

Diffusion, osmosis and active transport.

Explanation:

. Diffusion is the movement of substances from a region of high concentration to a region of low concentration across the membrane.

.Osmosis is the movement of water molecules from a hypotonic solution to a hypertonic solution across the membrane.

.Active transport is the movement of substances from a region of low concentration to a region of high concentration across the membrane with the utilization of energy.

Are the energy resources used at school renewable or nonrenewable

Answers

Answer:

Resources are characterized as renewable or nonrenewable; a renewable resource can replenish itself at the rate it is used, while a nonrenewable resource has a limited supply. Renewable resources include timber, wind, and solar while nonrenewable resources include coal and natural gas.

Explanation:

The aorta carries blood away from the heart and eventually branches into a large number of capillaries that distribute the blood to various body organs. If for an average person, the aorta has a radius of 1.2 cm and blood travels through it at a speed of 42 cm/s and the capillaries have an average radius of 5.0 10-4 cm and blood travels through them at a speed of 0.005 cm/s, determine the approximate number of capillaries in the human body.

Answers

Answer:

There are approximately 5 × 10¹⁰ capillaries in the human body

Explanation:

The motion of a fluid is such that the mass of the fluid flowing remains constant. This is known as the principle of conservation of mass in liquid flow.

The mass flow rate is the mass that passes a given point per unit time. According to the principle of conservation of mass, the flow rates at any two points must be equal, as long as no fluid is being added or taken away.

The principle gives rise tonthe continuity equation which is given below:

ρ₁A₁v₁ = ρ₂A₂v₂

Where ρ is density of fluid, A is internal area of the vessel or pipe the fluid is flowing through, v is velocity of the fluid

Since the density of blood is constant, the continuity equation above reduces to:

A₁v₁ = nA₂v₂

where A₁ is the area of the aorta, v₁ is velocity of bloodmin the aorta, n is number of capillaries, A₂ area of capillary, v₂ is velocity of blood in the capillary.

n = A₁v₁/A₂v₂

n = πr₁² × v₁ / πr₂² × v₂ where r₁ is radius of aorta and r₂ is radius of capillaries

n = (22/7 × 1.2² × 42) / (22/7 × 0.0005² × 0.005)

n = 4.8384 × 10¹⁰capillaries

Therefore, there are approximately 5 × 10¹⁰ capillaries in the human body

Please help me ASAP thank you

Answers

1. the sun 2. decomposers 3. i’m not sure 4. photosynthesis

What are the similarities between the coastal oceans and coral reefs

Answers

Answer:A coral reef is an underwater ecosystem characterized by reef-building corals. Reefs are formed of colonies of coral polyps held together by calcium carbonate. ... Unlike sea anemones, corals secrete hard carbonate exoskeletons that support and protect the coral.

Coastal oceans are commonly defined as the areas from the shoreline to the outer edge of the continental margin. They connect the continents to the open ocean and serve as a link for transporting organic and inorganic, natural and anthropogenic material from land to sea.

Coastal oceans in tropical areas have similar characteristics to their temperate counterparts. ... Coral reefs are biologically diverse and rich habitats that occur in relatively nutrient-poor environments along coastlines. Areas that have too many nutrients often harbor too much algal growth to sustain coral growth.,

Protein phosphatase 1 (PP1) catalyzes a reaction which yields products that regulate cell division; consequently, PP1 is a possible drug target to treat certain types of cancers. The PP1 enzyme cata- lyzes the hydrolysis of a phosphate group from myelin basic protein (MBP). The reaction is shown below:
PP1 MBP-phosphate-→ MBP+P,
The activity of PP1 was measured in the presence and absence of the inhibitor phosphatidic acid (PA). The concentration of PA was 300 nM. vo without PA vo with PA released . (mg/mL) mL"1 . min1) mL-1 . min-1) 0.00381 0.00620 0.00931 0.0140 0.010 0.0209 0.0335 0.0419 0.0838 0.015 0.025 0.050
(a) Use the data provided to construct a Lineweaver-Burk plot for the PP1 enzyme in the presence and absence of PA. What kind of inhibitor is PA?
(b) Report the KM and Vmax values for PP1 in the presence and absence of the inhibitor.

Answers

Answer:

Explanation:8iikk

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